Yuav ua li cas tawg los ntawm kev tsim cov teeb meem ntawm DHA

DHA algae roj, raws li polyunsaturated fatty acid muab rho tawm los ntawm marine microalgae, yog nplua nuj nyob rau hauv docosahexaenoic acid (DHA), uas muaj txiaj ntsig zoo rau tib neeg lub cev, thiab ua lub luag haujlwm tseem ceeb hauv kev loj hlob ntawm lub hlwb thiab retina. Vim nws txoj kev siv dav hauv cov khoom noj noj qab haus huv xws li cov mis mos thiab cov khoom noj rau niam, DHA algae roj kev lag luam tau tsim kho sai hauv xyoo tas los no. Lub sab sauv ntawm cov saw hlau muaj xws li kev cog qoob loo thiab sau cov microalgae, cov midstream tsom mus rau kev rho tawm thiab ua cov microalgae, thiab cov dej ntws qis yog ntim thiab muag cov roj algae.

Txawm hais tias kev tsim cov roj algae DHA muaj kev lag luam dav dav thiab muaj txiaj ntsig zoo rau kev noj qab haus huv, cov txheej txheem tsim cov roj algae DHA yog cov txheej txheem nyuaj thiab ilv. Txawm hais tias nrog kev nce qib ntawm cov thev naus laus zis, kev tsim khoom lag luam thiab cov khoom lag luam zoo tau txhim kho tas li, tseem muaj qee qhov teeb meem thiab teeb meem.

Raws li lub hom phiaj tseem ceeb ntawm kev tsim DHA, Schizochytrium schizochytrium los ntawm hiav txwv sib sib zog nqus thiab muaj kev loj hlob tshwj xeeb thiab kev loj hlob, uas nyuaj tshaj li hom kab mob. Ntxiv mus, kev ruaj ntseg thiab cov txiaj ntsig ntawm cov algae hom no cuam tshuam los ntawm ntau yam ntawm ib puag ncig, xws li kub, lub teeb, cov khoom noj khoom haus concentration, thiab lwm yam. Yog li ntawd, kev ruaj ntseg ntawm raw cov ntaub ntawv yog ib qho tseem ceeb heev rau zus tau tej cov DHA algae roj. Nyob rau tib lub sijhawm, cov txiaj ntsig thiab purity ntawm DHA tuaj yeem txhim kho los ntawm kev cog qoob loo thiab kev ua kom zoo ntawm algae hom. Txawm li cas los xij, cov txheej txheem no yuav tsum tau siv sijhawm ntev thiab ntau R&D peev, thiab muaj qhov tsis paub tseeb, xws li kev hloov pauv caj ces, uas yuav ua rau muaj kev yug me nyuam tsis ua haujlwm. Kev nrhiav tau, kev tshuaj ntsuam xyuas thiab kev ua kom zoo ntawm cov kab mob yog qhov nyuaj hauv DHA cov tshuab tsim khoom loj.

Algae roj DHA feem ntau suav nrog fermentation tswj technology thiab extraction thiab purification technology. Txoj kev fermentation yuav tsum tau ua nyob rau hauv ib tug fermenter. Kev tswj tsis tau xws li kub, av noo, lub teeb, thiab oxygen concentration thaum lub sij hawm fermentation yog ib qho tseem ceeb rau qhov zoo thiab tawm los ntawm cov khoom kawg. Txawm li cas los xij, kev tswj xyuas meej ntawm cov kev txwv no yuav tsum muaj cov cuab yeej siv siab thiab kev txhawb nqa, thiab yooj yim cuam tshuam los ntawm ib puag ncig sab nraud.

Qhov thib ob, rho tawm DHA los ntawm fermentation broth thiab purifying nws yog ib qho tseem ceeb txuas nyob rau hauv cov txheej txheem ntau lawm. Txoj kev rho tawm ib txwm muaj yuav muaj teeb meem xws li kev ua haujlwm qis thiab tus nqi siab. Txawm hais tias cov tshiab extraction thiab purification technologies (xws li hnyav-free extraction technology) tau txhim kho efficiency thiab purity, lawv muaj siab technical barriers thiab yuav tsum tau cov kws txawj thiab cov kws txawj.

Raws li cov khoom lag luam muaj txiaj ntsig, algae roj DHA yog qhov tseem ceeb rau kev tswj kom zoo. Thaum lub sij hawm tsim cov txheej txheem, nws yog ib qho tsim nyog yuav tsum tau ua raws li cov kev cai ntawm kev tswj xyuas zoo xws li GMP los xyuas kom meej qhov purity thiab kev nyab xeeb ntawm cov khoom. Txawm li cas los xij, qhov no tseem yuav nce cov nqi tsim khoom thiab cov nqi kuaj. Nyob rau tib lub sijhawm, tus nqi raw khoom, kev siv hluav taws xob, khoom siv poob qis, nqi zog, thiab lwm yam yog txhua yam tseem ceeb cuam tshuam rau cov nqi tsim khoom ntawm algae roj DHA. Yuav ua li cas txo cov nqi tsim khoom thaum ua kom cov khoom zoo yog qhov teeb meem tseem ceeb uas cov tuam ntxhab yuav tsum tau ntsib.

Hauv cov ntsiab lus, kev tsim cov roj algae DHA ntsib ntau yam teeb meem hauv cov khoom siv raw, cov txheej txheem tsim khoom, kev tswj kom zoo, kev tswj tus nqi, kev xav tau ntawm kev lag luam thiab kev sib tw, thiab cov cai thiab cov cai.

Txhawm rau kov yeej cov teeb meem no, HSF tau txuas ntxiv tsim kho tshiab thiab txhim kho thev naus laus zis. Nyob rau hauv txoj kev loj hlob thiab kev koom tes rau kaum xyoo, nws tau tob zuj zus zus tau tej cov strain breeding tswv yim, high-yield strain yug me nyuam thiab optimization technology, thiab sau fermentation technology kev thiab ntau lawm kev. Nrog kev pab los ntawm niaj hnub biotechnology, cov ntaub ntawv technology thiab engineering cov cuab yeej, pab neeg no tau ua tiav multi-link optimization thiab reconstruction nyob rau hauv cov kev tshawb fawb txoj kev thiab fermentation txheej txheem ntawm fermentation, thiab maj mam daws qhov teeb meem ntawm DHA algae roj industrialization.

Cov kab mob microbial yog lub hauv paus ntawm kev tsim khoom lom neeg fermentation. Yog li ntawd, txij li thaum tsim HSF Microbiological Laboratory nyob rau hauv 2012, pab neeg no tau ntev tau cog lus rau strain tshuaj ntsuam thiab lom fermentation technology. Tom qab ze li kaum xyoo ntawm kev txhim kho thev naus laus zis, nws tau tsim cov kev tshawb nrhiav thiab kev tshuaj ntsuam xyuas cov cuab yeej thiab cov cuab yeej cuab tam, suav nrog cov cuab yeej siv raws li cytometry technology, droplet microfluidics thev naus laus zis, microplate tshuaj ntsuam tshuab, thiab lwm yam, uas tuaj yeem txheeb xyuas cov kab mob tseem ceeb. Hauv kev xaiv DHA hom, xyoo 2016, 218 thawj hom kab mob hauv tsev thiab txawv teb chaws tau raug xaiv, thiab tom qab ntau txhiab qhov kev sim, Schizochytrium HSc-01 nrog cov DHA ntau lawm tau ua tiav cais. Kev loj hlob batch yog ruaj khov, cov kab mob sib xws thiab muaj xim tag nrho, thiab qhov siab tshaj plaws DHA cov ntsiab lus tuaj yeem ncav cuag 70%.

Thaum kuaj cov kab mob, pab pawg kuj tseem niaj hnub sau cov tshuab fermentation thiab kev tsim khoom hauv kev sim, tsim cov txheej txheem ntau lawm rau cov kab mob rov ua dua, thiab tau ntsib ntau yam kev rhuav tshem thiab kev tsim kho dua tshiab hauv rau xyoo, thiab tau ua 32 qhov kev txhim kho loj thiab me, thiab thaum kawg tau lees paub qhov fermentation. thiab cov txheej txheem ntau lawm.

Nyob rau hauv cov nqe lus ntawm optimizing fermentation tej yam kev mob thiab meej tswj, HSF siv advanced synthetic biology technology los soj ntsuam cov kab mob loj hlob thiab cov yam ntxwv tseem ceeb enzyme, thiab tsis tu ncua optimizes fermentation tej yam kev mob xws li fermentation kub, oxygen, pH nqi, substrate, thiab as-ham supplementation tus nqi thiab txoj kev. Nws siv cov khoom siv thoob ntiaj teb siab tshaj plaws tsis siv neeg fermentation cov cuab yeej los tswj cov kab mob loj hlob thiab kev hloov pauv, ua tiav cov khoom lag luam fermentation siab, thiab tau ua kom muaj kev cuam tshuam loj hauv kev lag luam ntawm microbial fermentation.

Hais txog kev sib cais ntawm cov khoom siv thev naus laus zis, HSF feem ntau tsom rau ob yam ntawm kev tshawb fawb: ib qho yog kev sib cais ntawm lipid as-ham, thiab lwm yam yog kev sib cais ntawm cov pa phem. Hauv kev sib cais ntawm DHA, cov qauv kev ua haujlwm ntawm DHA algae roj yog tiv thaiv kom siab tshaj plaws, thiab kev cuam tshuam ntawm aldehydes thiab ketones hauv DHA algae roj ntawm tsw yog txo kom ntau li ntau tau, uas txo cov seem hauv cov khoom thiab cov tshuaj tiv thaiv tsim. Cov extracted DHA algae roj muaj siab purity thiab ruaj khov batch ntsiab lus. Cov ntsiab lus DHA nyob deb tshaj li cov qauv ntawm kuv lub teb chaws thiab Europe thiab Tebchaws Meskas, thiab cov ntsiab lus EPA yog qhov tsawg heev, uas yog qhov tsim nyog rau cov khoom noj mis mos ~

Pib txij xyoo 2022, Haisfu tau nqis peev 110 lab los tsim cov kab ntau lawm uas tuaj yeem ua kom pom zoo hais txog Haisfu's R&D thev naus laus zis. Cov kab fermentation no coj los ua ke kev txawj ntse ntawm Mrs. Haisfu rau ntau tshaj kaum xyoo. Nws yog qhov tsim nyog tshaj plaws rau kev lag luam ntawm DHA algae roj nyob rau theem no, nrog rau qib siab tshaj plaws ntawm kev nce qib thiab ntau tshaj txhiab-tuj ntau lawm kab.

Txawm hais tias DHA algae roj tuaj yeem ncaj qha ntxiv lossis noj hauv cov ntawv thov tiag tiag, nws qhov kev siv yuav yooj yim heev thiab qhov ntau npaum li cas yuav tsis yooj yim los tswj. Raws li cov kws tsim khoom lag luam uas nkag siab cov neeg siv khoom zoo dua, HSF tau sim nws qhov zoo tshaj plaws los txuas ntxiv cov ntawv thov ntawm DHA algae roj thiab tsim ntau yam khoom lag luam rau cov neeg siv khoom. Nrog kev txhawb nqa ntawm VegeLipi® thev naus laus zis, nws kuj tau tsim DHA algae roj hmoov uas siv tau yooj yim dua. Los ntawm kev ua haujlwm roj embedding thev naus laus zis, nws zoo kawg nkaus daws cov teeb meem ntawm DHA algae roj, xws li bioavailability tsis zoo, tsis muaj dej solubility, yooj yim oxidation, saj tsis zoo, nyuaj hauv premixing, thiab nyuaj hauv kev thauj mus los. HSF's microencapsulation thev naus laus zis tau txuas ntxiv polished nyob rau hauv qhov tseeb ntau lawm thiab siab dua yuav tsum tau muab tso rau pem hauv ntej los ntawm cov neeg siv khoom. Nws tau tsim kom muaj kev tshawb fawb tus kheej tsim emulsification system thiab sib tw cov khoom lag luam zoo tshaj plaws kev daws teeb meem raws li kev thov sib txawv.