CoQ10 fermented li cas?

Coenzyme Q10 (coenzyme Q10, CoQ10) yog cov roj-soluble quinone compound. Raws li ib txwm tshwm sim coenzyme, CoQ10 yog ib qho tseem ceeb antioxidant thiab electron transporter rau tib neeg lub cev. Nws yog ib qho tseem ceeb hauv kev ua pa, uas tuaj yeem txhim kho cov khoom ntawm intracellular ATP, txo cov dawb radicals hauv lub cev, tiv thaiv oxidation, pab ncua kev laus, thiab txhim kho lub cev tiv thaiv kab mob. Coenzyme Q10 tau siv dav hauv kev lag luam khoom noj, tshuaj, tshuaj pleev ib ce, thiab tsiaj txhu pub, thiab kev lag luam xav tau thiab kev siv tau nce ntxiv.

Muaj peb txoj hauv kev tseem ceeb rau kev tsim cov coenzyme Q10: ib qho yog txoj kev rho tawm los ntawm cov nroj tsuag thiab tsiaj cov ntaub so ntswg; qhov thib ob yog txoj kev siv tshuaj lom neeg; thiab qhov thib peb yog txoj kev rho tawm los ntawm microbial fermentation. Tam sim no, txoj hauv kev tseem ceeb rau kev tsim cov coenzyme Q10 yog txoj kev fermentation, uas tuaj yeem tsis tsuas yog nthuav dav cov nplais, tab sis kuj muaj qhov zoo ntawm cov khoom tsim tau.

Kev xaiv hom kab mob

Txawm hais tias cov ntsiab lus ntawm coenzyme Q10 nyob rau hauv microbial hlwb nyob rau hauv cov xwm yog siab, nws cov khoom fermentation yog ib tug sib tov ntawm ntau coenzyme Q10 homologs, uas ua rau tus nqi siab rau purification ntawm coenzyme Q10. Yog li ntawd, nyob rau hauv fermentation zus tau tej cov coenzyme Q10, kev xaiv ntawm ntau lawm hom yog thawj qhov teeb meem. Cov ntsiab lus ntawm coenzyme Q10 hauv cov kab mob photosynthetic (tom qab no hu ua PSB) feem ntau yog siab. Raws li kev faib tawm, PSB belongs rau qhov kev txiav txim ntawm Rhodospirillales, qhov kev txiav txim ntawm Eubacteria, qhov kev txiav txim ntawm cov kab mob, qhov kev txiav txim ntawm Rhodospirillales, thiab kev txiav txim ntawm Chlorobacteria. Cov qub tau muab faib ua Rhodothiobacteriaceae thiab Rhodospirillaceae. Yog li ntawd, cov kab mob Rhodospirillaceae yog ib qho kev xaiv zoo tshaj plaws rau coenzyme Q10- ua hom. Lub peev xwm tsim cov coenzyme Q10 ntawm cov tsiaj qus hom tsis tuaj yeem ua tau raws li cov kev xav tau ntau lawm, thiab lawv tuaj yeem hloov kho caj ces siv cov qauv mutagenesis thiab genetic engineering cov txheej txheem.

Optimization ntawm fermentation tej yam kev mob

Ntxiv nrog rau kev siv cov txheej txheem tswj cov txheej txheem metabolic los xaiv cov txiaj ntsig zoo los yog tsim cov kab mob sib txuas kom nce fermentation yield, optimizing fermentation tej yam kev mob ntawm cov kab mob ntau lawm kuj yog ib qho tseem ceeb thiab muaj txiaj ntsig txoj hauv kev kom fermentation tawm los.

• Optimization ntawm kab lis kev cai nruab nrab

Nyob rau hauv lub fermentation ntawm coenzyme Q10, fermentation optimization thwmsim yog nqa tawm los ntawm kev xaiv cov pa roj carbon, nitrogen qhov chaw, loj hlob yam, inorganic ntsev, thiab lwm yam los ntawm ntau qhov chaw los txiav txim seb muaj pes tsawg leeg ntawm kab lis kev cai nruab nrab. Cov kev tshawb fawb tau pom tias cov hlau ions, tshwj xeeb tshaj yog Mg2+, Fe2+, thiab Mn2+, txhawb kev fermentation ntawm coenzyme Q10. Tsis tas li ntawd, precursors tuaj yeem ua rau muaj txiaj ntsig zoo ntawm cov khoom lag luam thiab, nyob rau hauv qee qhov xwm txheej, tseem tuaj yeem tswj hwm cov khoom lag luam anabolism. Nws tau raug tshaj tawm tias cov precursors ntxiv rau fermentation ntawm coenzyme Q10 muaj xws li p-hydroxybenzoic acid, mevalonate, isopentanol, thiab geraniol.

• Optimization ntawm kab lis kev cai

ib. Kev ntxhov siab - aeration

Cov teebmeem ntawm agitation-aeration ntawm cov txiaj ntsig ntawm coenzyme Q10 sib txawv nyob ntawm seb qhov ntau lawm. Ib qho yog tias nws tuaj yeem txhawb kev tsim cov fermentation ntawm coenzyme Q10; Lwm qhov yog tias nws muaj cov nyhuv inhibitory. Cov kev tshawb fawb tau pom tias cov cytoplasmic membrane ntawm cov kab mob loj hlob nyob rau hauv cov kev txwv oxygen yog tsim tau zoo thiab muaj cov txheej txheem ntau, thiab lub chaw photoreaction ntawm PSB nyob ntawm no, uas yuav ua rau muaj cov ntsiab lus siab dua ntawm coenzyme Q10 dua li cov kab mob loj hlob hauv cov pa oxygen txaus. muab tej yam kev mob.

b. Teeb

Cov kab mob Rhodospirillaceae tuaj yeem ua obligate anaerobic bacterial photosynthesis thiab aerobic respiration thiab fermentation. Tsheb thiab Excell tau tshaj tawm tias PSB muaj kev tsim cov coenzyme Q10 nyob rau hauv lub teeb anaerobic tej yam kev mob, tab sis thaum nws hloov mus rau tsaus aerobic kab lis kev cai, cov ntau lawm yuav poob sharply.

c. pH

Thawj tus nqi pH yuav cuam tshuam rau tus nqi ntawm cov kab mob siv cov substrate thiab cov txheej txheem ntawm lub cev, yog li cuam tshuam rau kev loj hlob ntawm cov kab mob thiab cov kev hloov hauv metabolites. Wang Genhua et al. tau ua ib qho kev sim co khob ntawm Rhizobium leguminosarum thiab pom tias cov kab mob tsim coenzyme Q10 ntau dua nyob rau hauv acidic tej yam kev mob dua li nyob rau hauv alkaline tej yam kev mob. Tus nqi ntawm intracellular coenzyme Q10 yog siab tshaj ntawm pH 5.

d. Lub sijhawm cog qoob loo

Yoshida et al. pom tias cov ntsiab lus ntawm coenzyme Q10 yog siab dua thaum cov kab mob nyob rau hauv lub sij hawm thaum ntxov ntawm lub sij hawm ruaj khov. Zhu Xufen et al. kuj ntseeg hais tias cov ntsiab lus ntawm CoQ10 nyob rau hauv cov kab mob txuas ntxiv nrog kev nce ntawm lub sij hawm cog qoob loo, nce mus txog qib siab tshaj plaws nyob rau theem pib thiab nruab nrab ntawm lub sijhawm ruaj khov, thiab tom qab ntawd pib poob qis.

• Optimization ntawm cov qauv kev cob qhia

Cov qauv kab lis kev cai tau muab faib ua batch fermentation, pub-batch fermentation, pub-batch fermentation, nruam fermentation, thiab lwm yam. Cov kab lis kev cai sib txawv muaj qhov zoo thiab qhov tsis zoo, thiab hom kab lis kev cai tuaj yeem cuam tshuam ncaj qha rau kev tsim cov coenzyme Q10. Tam sim no, kev noj zaub mov-batch kab lis kev cai yog qhov zoo dua. Fed-batch tuaj yeem zam cov metabolism-sib sau ua ke thiab tau txais cov xov tooj ntawm tes ntau dua, ua kom cov txheej txheem fermentation ntev dua, thiab khaws cov khoom lag luam ntau. Los ntawm kev kawm txog kev ua pa ntawm Coenzyme Q10- tsim cov kab mob, kev sib raug zoo ntawm cov piam thaj metabolism thiab Coenzyme Q10 ntau lawm, thiab kev soj ntsuam kinetic ntawm cov pa oxygen thiab cov piam thaj, nws tau pom tias kev tswj cov piam thaj qis thaum lub sijhawm cov txheej txheem fermentation yog qhov tsim nyog rau kev tsim cov khoom. Yog li ntawd, nws kuj yog Fed-batch kab lis kev cai uas tuaj yeem siv tau. Kev tshawb fawb ntawm radioactive rhizobia pom tias sib xyaw pub-batch fermentation nrog sucrose thiab ib qho tsim nyog ntawm cov pob kws-steked cawv tuaj yeem ua rau muaj kev tsim cov coenzyme Q10, thiab nws cov ntsiab lus intracellular coenzyme Q10 nce 33%.

Extraction ntawm Coenzyme Q10

Txij li thaum Coenzyme Q10 yog cov khoom lag luam intracellular tau los ntawm microbial fermentation, phab ntsa ntawm tes yuav tsum tau cuam tshuam ua ntej thaum lub sij hawm rho tawm. Yog li, kev cuam tshuam ntawm tes yog ib qho ntawm cov kauj ruam tseem ceeb hauv kev rho tawm Coenzyme Q10, uas cuam tshuam ncaj qha rau kev ua haujlwm lom neeg, tawm los, thiab tus nqi ntawm Coenzyme Q10. Tom qab tshuaj xyuas cov ntaub ntawv, peb pom tias muaj ntau txoj hauv kev rau rho tawm Coenzyme Q10. Kab lus no piav qhia thiab sib piv ntau txoj hauv kev uas feem ntau siv rho tawm.

• Saponification extraction txoj kev

Txoj kev saponification extraction hloov pauv ntau ntawm cov dej-soluble Cheebtsam xws li fatty acid glycerides thiab ntau yam dawb fatty acids uas muaj nyob rau hauv Coenzyme Q10 extract mus rau lub aqueous theem, thaum cov roj-soluble Coenzyme Q10 tseem nyob rau hauv cov organic theem, thiab Tom qab ntawd muab rho tawm siv cov kuab tshuaj organic. , yog li txhim kho nws purity thiab extraction muaj peev xwm. Cov txheej txheem saponification extraction feem ntau suav nrog cov cawv-alkali saponification extraction method thiab alkali saponification extraction method.

ib. Cawv-alkali saponification extraction method

Txoj kev dav dav ntawm cawv-alkali saponification extraction method yog: thawj zaug ntxiv pyrogallic acid nrog ib feem ntawm kwv yees li 7% rau cov kab mob hlwb, ces maj mam ntxiv 1.3 ~ 1.5 npaug ntawm sodium hydroxide-ethanol tov, do, thiab ces ntxiv. n-hexane. Reflux rau extraction thiab sai txias rau chav tsev kub. Tom qab ntawd siv tus nqi tsim nyog ntawm cov roj av ether los rho tawm ntau zaus, ntxuav nrog dej kom txog thaum nruab nrab, mloog zoo nrog dej, lub tshuab nqus tsev thiab cov rotary evaporate kom tshem tawm cov organic hnyav, thiab thaum kawg yaj nrog ethanol kiag li. Nyob rau hauv txoj kev cawv-alkali saponification, vim muaj ethanol, saponification mus sij hawm ntev yuav ua rau kev hloov ntawm methoxy pawg hauv coenzyme Q10 thiab ethoxy pawg hauv ethanol, uas ua rau ib leeg lossis ob npaug ethoxy derivatives.

b.Alkali saponification extraction method

Txoj kev dav dav ntawm alkali saponification extraction method yog: thawj zaug ntxiv cov dej acidic rau cov kab mob, cua sov, thiab reflux hauv dej da dej rau 3 teev, maj mam ntxiv sodium hydroxide diluted mus rau ntau yam, tom qab ntawd reflux rau qee lub sijhawm, thiab txias sai sai. chav kub. Tom qab ntawd ntxiv leaching nrog cov kuab tshuaj organic 2 mus rau 3 zaug, coj cov supernatant thiab ntxuav nws nrog dej kom txog thaum nruab nrab, ces muab dej thiab mloog zoo, lub tshuab nqus tsev concentration, thiab rotary evaporation kom tshem tawm cov organic hnyav, thiab thaum kawg dissolve nws nrog kiag li ethanol. Piv nrog rau alkaline saponification extraction txoj kev, txawm hais tias cov tshuaj tsim ib qho emulsification tshwm sim, alkali saponification extraction txoj kev muaj ntau dua extraction npaum li cas ntawm coenzyme Q10 thiab tsim tsawg impurities.

• Cell cuam tshuam txoj kev rho tawm

ib. Organic hnyav stirring thiab crushing extraction txoj kev

Txoj kev dav dav ntawm cov organic hnyav stirring thiab crushing extraction txoj kev yog: thawj zaug ntxiv cov kab mob ntub dej thiab acetone rau hauv lub beaker, do nrog ib tug sib nqus stirring qws, centrifuge thiab ncuav tawm lub supernatant, ces siv ib tug teb evaporator kom mloog zoo rau supernatant ntawm 50 degree. , thiab tom qab ntawd ntxiv roj av Extract nrog ether los yog n-hexane, thiab tom qab ntawd mloog zoo rau cov extract nrog lub evaporator teb rau ib kua tuab. Thaum kawg, ntxiv cov ethanol kiag li thiab khov rau hauv lub tub yees kom cais cov impurities xws li cov cholesterol. Lim thiab rov qab cov lim.

b. Kev sib tsoo, crushing, thiab extraction method

Txoj kev dav dav ntawm kev sib tsoo thiab crushing extraction txoj kev yog: ua ntej, coj cov kab mob ntub dej rau hauv ib lub mortar, ntxiv me me ntawm quartz xuab zeb, acetone, thiab antioxidants kom zom sai sai, zom rau qee lub sij hawm, ces centrifuge, ncuav lub supernatant tom qab. centrifugation, thiab tom qab ntawd ntxiv roj av Extract nrog ether los yog n-hexane, thiab tom qab ntawd mloog zoo rau cov extract nrog lub rotary evaporator rau cov kua tuab. Thaum kawg, ntxiv cov ethanol kiag li thiab khov rau hauv lub tub yees kom cais cov impurities xws li cov cholesterol. Lim thiab rov qab cov lim.

c. Ultrasonic crushing thiab extraction txoj kev

Txoj kev dav dav ntawm ultrasonic crushing thiab extraction yog raws li hauv qab no: ua ntej, ntxiv acetone rau cov kab mob ntub dej los npaj cov kab mob tshem tawm, tom qab ntawd siv ultrasonic cell cuam tshuam los tsoo cov kab mob hauv cov dej khov da dej, thiab thaum kawg centrifuge rau extraction.

Lub luag haujlwm ntawm dawb radical scua pauj

Coenzyme Q yog lub zog hloov pauv hauv mitochondria ntawm cov hlwb. Nws koom nrog "tricarboxylic acid voj voog" los ntawm kev hloov thiab xa cov hluav taws xob los tsim ATP (adenosine triphosphate), uas yog, lub zog tseem ceeb rau cell metabolism. Cov kev sim tau pom tias tom qab coenzyme Q10 hauv lub cev dhau los ua cawv, nws tuaj yeem cuam tshuam ncaj qha nrog peroxide dawb radicals kom rov tsim VE, thiab nws tus kheej thiab sib koom ua lub luag haujlwm ntawm antioxidant.

Kev poob qis hauv kev tiv thaiv kab mob nrog kev laus yog qhov tshwm sim ntawm cov dawb radicals thiab cov tshuaj tiv thaiv dawb radical. Coenzyme Q10, raws li lub zog antioxidant, tuaj yeem cuam tshuam qhov sib txawv ntawm cov receptors thiab cov hlwb ntawm lub cev tiv thaiv kab mob los ntawm cov dawb radicals ib leeg lossis ua ke nrog cov vitamin B6 (pyridoxine), thiab muaj kev hloov pauv ntawm cov kab mob microtubule ntsig txog kev ua haujlwm, txhim kho kev tiv thaiv kab mob. , thiab ncua kev laus.

Lub luag haujlwm ntawm kev tiv thaiv daim tawv nqaij

Coenzyme Q10 nkag mus rau txheej txheej ntawm daim tawv nqaij kom tsis muaj zog ntawm cov tshuaj tiv thaiv oxidation ntawm photons thiab tuaj yeem qhib cov phosphorylated tyrosine kinases nrog kev pab ntawm tocopherol. Tiv thaiv oxidative puas tsuaj rau DNA, inhibit qhov kev qhia ntawm collagenase nyob rau hauv tib neeg daim tawv nqaij fibroblasts nyob rau hauv ultraviolet lub teeb, thiab tiv thaiv daim tawv nqaij los ntawm kev puas tsuaj. Kev tshawb fawb dav dav qhia tau hais tias coenzyme Q10 inhibits lipid peroxidation, txo cov tiam ntawm cov dawb radicals, tiv thaiv SOD active chaw thiab nws cov qauv los ntawm dawb radical oxidative puas, tsub kom cov kev ua ntawm enzymes xws li SOD nyob rau hauv lub cev, inhibits cell apoptosis induced los ntawm oxidative kev nyuaj siab. , thiab muaj cov nyhuv antioxidant tseem ceeb.

Kev tiv thaiv kab mob

Coenzyme Q10 yog lub ntuj antioxidant thiab cell metabolism pib tsim los ntawm cov hlwb lawv tus kheej. Nws muaj kev ua haujlwm ntawm kev tiv thaiv thiab rov ua kom muaj kev ncaj ncees ntawm cov txheej txheem ntawm cov kab mob lom neeg thiab ua kom ruaj khov ntawm daim nyias nyias. Nws yog ib qho kev tiv thaiv kab mob tsis tshwj xeeb ntawm lub cev, yog li nws qhia tau zoo heev los tiv thaiv qaug zog. Coenzyme Q10 ua kom cov hlwb nyob hauv lub xeev zoo thiab noj qab haus huv, yog li lub cev muaj zog thiab lub hlwb muaj zog.

Txhim kho cov leeg nqaij zog

Cov kev tshawb fawb tau pom tias kev ntxiv ntawm 100 ~ 150mg / hnub ntawm coenzyme Q10 tuaj yeem txhim kho cov neeg mob ntawm cov leeg nqaij dystrophy. Kev tawm dag zog lub cev ua rau txo cov ntshav cov ntsiab lus ntawm coenzyme Q10, thiab kev noj tshuaj txhua hnub ntawm 60mg tuaj yeem txhim kho kev ua haujlwm ntawm cov neeg ncaws pob. Ntau tus neeg rog rog muaj qis heev ntawm Coenzyme Q10, thiab kev ntxiv tuaj yeem pab lawv poob phaus. Qhov no yog vim Coenzyme Q10 tuaj yeem ua kom cov metabolism hauv cov rog, muab cov ceg thiab lub hlwb nrog lub zog txaus thiab ua kom muaj zog.

(1) Cov zaub mov

Coenzyme Q10 kuj tau siv dav hauv kev lag luam khoom noj. Nws ntxiv rau qee yam khoom noj, xws li khoom noj khoom haus, dej haus, qhob cij, khoom qab zib, thiab lwm yam, kom lawv cov khoom noj muaj txiaj ntsig. Tsis tas li ntawd, nws kuj tseem siv los txhim kho cov khoom antioxidant ntawm cov zaub mov, uas pab tiv thaiv cov zaub mov tsis txhob lwj thiab lwj.

(2) Kev kho mob teb

Coenzyme Q10 kuj tau siv dav hauv kev kho mob thiab ua lub luag haujlwm tseem ceeb hauv kev saib xyuas tib neeg kev noj qab haus huv. Nws yog siv los kho ntau yam kab mob xws li kab mob plawv, mob qog noj ntshav, kab mob neurodegenerative, thiab lwm yam. Kev tshawb fawb pom tias Coenzyme Q10 tuaj yeem pab txhim kho lub plawv mob, txo qis qaug zog, txhim kho kev tiv thaiv kab mob, thiab muaj kev tiv thaiv kev laus.

(3) Cosmetic teb

Coenzyme Q10 kuj tseem siv rau hauv ntau yam tshuaj pleev ib ce vim nws cov tshuaj tua kab mob antioxidant thiab tiv thaiv kab mob. Cov khoom no tuaj yeem pab qeeb ntawm daim tawv nqaij laus thiab txhim kho daim tawv nqaij radiance thiab elasticity. Coenzyme Q10 yog ib qho tseem ceeb muaj nyob rau hauv ntau yam khoom tu tawv nqaij thiab kev kho kom zoo nkauj.

(4) Tsiaj noj zaub mov

Hauv kev lag luam tsiaj txhu, Coenzyme Q10 kuj tseem siv rau hauv kev pub. Coenzyme Q10 tau ntxiv rau kev pub tsiaj txhu thiab nqaij qaib xws li npua thiab qaib los txhawb lawv txoj kev loj hlob thiab kev loj hlob, txhim kho cov nqaij thiab qe zoo, thiab txhim kho cov kab mob. Nyob rau tib lub sijhawm, nws tseem tuaj yeem txhim kho kev ua haujlwm ntawm kev ua me nyuam thiab ua rau kom muaj kev loj hlob ntxiv. Cov kev tshawb fawb tau pom tias coenzyme Q10 tuaj yeem nce tus nqi loj hlob thiab tawm los ntawm cov tsiaj thiab txhim kho lawv lub peev xwm tiv thaiv kab mob. Yog li ntawd, nws yog dav siv nyob rau hauv cov tsiaj yug tsiaj pub los txhim kho kev lag luam.

Txhawm rau txhim kho bioavailability ntawm Coenzyme Q10, nws yog ib qho tseem ceeb uas yuav tau xaiv cov qauv tsim nyog raws li cov kev xav tau sib txawv, ua kom muaj kev tso tawm zoo dua thiab ua haujlwm hauv lub cev. Cia siab rau peb cov kev sib cais thiab kev siv tshuab thev naus laus zis, HSF Biotech tsim Coenzyme Q10 siv cov dej txias dispersion microencapsulation tshuab. Qhov no ua kom ruaj khov kev ua haujlwm, kev tswj xyuas nruj ntawm cov kab paug paug, thiab cov dej ntws zoo. Nws sai sai disperses nyob rau hauv dej txias, ua rau nws haum rau ntau yam kev siv xws li cov dej haus, gummies, thiab premixes. Nws tuaj yeem ua ke nrog cov vitamin E / cov roj cog thiab siv dav hauv cov khoom noj khoom haus thiab cov tshuaj ntsiav, ntawm lwm yam khoom.

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