Tsis ntev los no, thoob ntiaj teb nto moo Plant Cell tau tshaj tawm txoj kev tshawb fawb online hu ua&";Leucine-Rich Rov Qab Tau Txais Tus Kinase Signaling Pathways los ntawm BAK1-Sib cuam tshuam Receptor-Zoo li Kinase 3 Chimera" los ntawm Michael Hothorn' pab pawg los ntawm Department of Botany thiab Plant Biology, University of Geneva, Switzerland. Kev sib deev thesis. Cov txheej txheem ua kom muaj zog ntawm BAK1 kev sib cuam tshuam receptor kinase 3 chimera ntawm leucine-nplua nuj rov ua tus txais tos kinase teeb liab qhia tau pom.
Cov nroj tsuag' lub cim tshwj xeeb ntawm kev sib koom ua ke-kev ua haujlwm ntawm SERK tau khaws cia hauv ntau LRR-RKs, xws li LRR-RK HAESA, uas nws txoj haujlwm suav nrog tswj kev tso cov paj ntoo hauv Arabidopsis thaliana los ntawm kev cuam tshuam nrog peptide hormone IDA. SERK-dependent mitogen-activated protein kinase (MAPK) cov paib qhia txog txoj hauv kev cuam tshuam nrog LRR-RK ERECTA (ER) thiab nws cov noob homologous ERECTA-LIKE1 (ERL1) thiab ERL2, thiab ua lub luag haujlwm sib txawv hauv kev cog ntoo. Kev txheeb xyuas ntawm kev sib koom ua ke thiab ligand-ywj siab sib cuam tshuam ntawm LRR cov kab hluav taws xob txuas ntxiv ntawm ob tsob ntoo cog cov cim qhia kom peb tshawb xyuas seb puas muaj cov protein chimera nruab nrab ntawm BIR3 extracellular domain thiab cytoplasmic domains ntawm ntau yam receptor kinases yog ua tau. nyuaj. Txawm hais tias muaj qhov sib txawv tseem ceeb ntawm LRR-RKSERK thiab BIR-SERK txoj haujlwm, cov kws tshawb fawb cov ntaub ntawv qhia tias ntau yam ntawm oBIR3-iLRR-RK chimeras ua haujlwm hauv cov nroj tsuag (Daim duab 1).
Tus txais kinases ntawm leucine-nplua nuj extracellular rov ua dua qub (LRR-RKs) yog pawg loj tshaj plaws ntawm cov paib qhia cov protein hauv cov nroj tsuag. LRR-RKs tuaj yeem nkag siab txog cov lwg me me, peptides lossis protein ligands, thiab tuaj yeem ua haujlwm los ntawm ligand-induced cuam tshuam nrog somatic embryogenesis receptor-like kinase (SERK) co-receptor kinase. Peb cov kev tshawb fawb yav dhau los tau qhia tias SERK tseem tuaj yeem cuam tshuam nrog BAK1 li receptor kinase 3 (BIR3) los tsim cov kev sib koom ua ke, ligand-ywj siab ua. Ntawm no, cov kws tshawb fawb tau tshaj tawm tus neeg txais chimera, uas nyob rau sab nraud LRR sau ntawm BIR3 tau sib xyaw nrog cytoplasmic kinase sau ntawm SERK-nyob LRR-RKs. Thaum tsis muaj kev txhawb nqa ligand, BRASSINOSTEROID INSENSITIVE1, HAESA, thiab ERECTA Source-derived SERK co-receptors tsim cov txheej txheem nruj. Cov suab paj nruag no tau hais qhia nyob rau hauv kev tswj hwm ntawm cov neeg txhawb nqa endogenous ntawm LRR-RKs thiab ua rau lub luag haujlwm muaj txiaj ntsig kom tau txais cov phenotype ntawm brassinosteroids, poob paj, thiab cov qauv hauv plab. Qhov tseem ceeb, BIR3-GSO1/SGN3 chimera tuaj yeem ua ib feem ntxiv cov phenotype tsim los ntawm sgn3 Karnofsky band, uas qhia tias SERK protein kuj tseem ua kom muaj kev txhawb nqa GSO1/SGN3. Feem ntau, peb cov txheej txheem kev tsim cov protein tuaj yeem siv los piav qhia lub cev lub luag haujlwm ntawm LRR-RKs thiab txiav txim siab lub tshuab txais kev ua haujlwm hauv ib qho kev hloov pauv ib leeg (Daim duab 2).
Hauv cov ntsiab lus, peb muaj kev yooj yim, Lego-hom sib dhos thiab cov kab tsim nyog tsim nyog rau BIR3 chimera tam sim no tso cai rau tus xeeb ceem ntawm cov menyuam ntsuag LRR-RKs nrog qhov tsis paub/tsis paub meej poob-ntawm-kev ua haujlwm phenotypes thiab kev tshuaj xyuas ntawm lawv cov hauv paus ua haujlwm. BIR3 protein chimeras kuj tseem tuaj yeem siv rau kev tshuaj ntsuam xyuas biochemical lossis caj ces, qhov twg yog qhov ua tau zoo ntawm tus neeg txais khoom.