Tsis ntev los no, Cov Khoom Noj Enzyme Engineering Innovation Team ntawm lub koom haum ntawm Kev Ua Liaj Ua Teb Cov Khoom Ua Liaj Ua Teb, Suav Academy ntawm Kev Tshawb Fawb Txog Kev Ua Liaj Ua Teb tau txheeb xyuas hom tshiab.ferulic acidesterase Raws li FAE los ntawm Allobacterium serratia plab hnyuv Substrate khi thiab catalytic mechanisms muab kev tshawb fawb hauv paus rau kev nkag siab tob txog kev noj zaub mov fiber ntau metabolism hauv txoj hnyuv, nrog rau kev taw qhia rau kev noj zaub mov zoo thiab kev kho mob ntawm cov hnyuv enzymes. Cov txiaj ntsig kev tshawb fawb cuam tshuam tau luam tawm nyob rau hauv Phau Ntawv Qhia Txog Kev Ua Liaj Ua Teb thiab Khoom Noj Chemistry nyob rau hauv daim ntawv ntawm ib daim ntawv npog nrog lub npe" Lub α-Helical Cap Domain ntawm Novel Esterase los ntawm Gut Alistipes shahii Shaping the Substrate-Binding Pocket [ GG] quot;.
Raws li tus kws tshawb fawb Xin Fengjiao, tib neeg txoj hnyuv plab yog tus choj tseem ceeb uas ua rau cov khoom noj metabolism thiab kev tswj hwm lub cev, thiab ua lub luag haujlwm tseem ceeb hauv tib neeg kev noj qab haus huv thiab kab mob. Cov nroj tsuag tshwj xeeb tuaj yeem tso cov ferulic acid los ntawm kev noj zaub mov fiber ntau los ntawm kev encoding tshwj xeeb ferulic acid esterase, thiab tom qab ntawd ua haujlwm xws li tiv thaiv oxidation, tiv thaiv kab mob, tiv thaiv qog, txhim kho cov kab mob plawv, thiab tswj lub hlwb kev paub. Nyob rau hauv txoj kev tshawb no dhau los, xya ferulic acid esterase noob tau tswj hwm siv cov kab mob vitro fecal fermentation. Nyob rau hauv txoj kev tshawb no, kev qhia, purification thiab enzymatic zog ntawm ferulic acid esterase muab tau los ntawm S. plab hnyuv kab mob. Qhov zoo tshaj plaws kub ntawm cov enzyme yog 40 ° C, qhov zoo pH yog 8.5, thiab nws muaj ib tug siab substrate nyiam rau luv-chain fatty acid esters. Txoj kev tshawb no ntxiv soj ntsuam peb-dimensional qauv ntawm lub enzyme, qhia tias tus cim"cap" domain plays lub luag haujlwm tseem ceeb hauv enzyme' s substrate khi thiab catalysis.
